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Anti-Ribophorin I Rabbit Monoclonal Antibody
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Application 
| WB, IHC, FC |
|---|---|
| Primary Accession | P04843 |
| Host | Rabbit |
| Isotype | Rabbit IgG |
| Reactivity | Rat, Human |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-Ribophorin I Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat. |
| Gene ID | 6184 |
|---|---|
| Other Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1 (HGNC:10381) |
| Calculated MW | 68569 Da |
| Application Details | WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:100 |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: 32R32 |
| Immunogen | A synthesized peptide derived from human Ribophorin I |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | RPN1 (HGNC:10381) |
|---|---|
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:E2RQ08}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:E2RQ08}. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Tissue Location | Expressed in all tissues tested. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 370.00
Cat# ABO16761
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